What's New

Fibromyalgia


Arthritis Research & Therapy 2010, 12:R17doi:10.1186/ar2918

 

Research article

 

Mitochondrial dysfunction and mitophagy activation in blood mononuclear cells of fibromyalgia patients: implications in the pathogenesis of the disease

 

Mario D Cordero (1,2,3*) , Manuel De Miguel (3*), Ana M Moreno Fernández (3) , Inés M Carmona López (3) , Juan Garrido Maraver (1,2) , David Cotán (1,2) , Lourdes Gómez Izquierdo (4) , Pablo Bona (5) , Francisco Campa (5,6) , Pedro Bullon (7) , Plácido Navas (1,2) and José A Sánchez Alcázar (1,2)

 

  1. Centro Andaluz de Biología del Desarrollo (CABD), Universidad Pablo de Olavide-CSIC, Ctra. de Utrera, km. 1, ISCIII, Sevilla 41013, Spain
  2.   Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Universidad Pablo de Olavide-CSIC, Ctra. de Utrera, km. 1, ISCIII, Sevilla 41013, Spain
  3. Dpto. Citología e Histología Normal y Patológica, Facultad de Medicina. Universidad de Sevilla, Avda. Dr. Fedriani s/n, Sevilla 41009, Spain
  4. Departamento de Anatomía Patológica. Hospital Virgen del Rocío, Sevilla 41013, Spain
  5. Dpto. de Medicina, Facultad de Medicina. Universidad de Sevilla, Avda. Dr. Fedriani s/n, Sevilla 41009, Spain
  6. Distrito Sanitario Sevilla Sur., Facultad de Odontología, Universidad de Sevilla, Campus de los Perdigones, C/Avicena s/n, Sevilla 41009, Spain
  7. Departamento de Periodontología, Facultad de Odontología, Universidad de Sevilla, Campus de los Perdigones, C/Avicena s/n, Sevilla 41009, Spain

* Contributed equally

 

Article URL : http://arthritis-research.com/content/12/1/R17

 

Published: 28 January 2010

 

© 2010 Cordero et al. , licensee BioMed Central Ltd.
This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0/),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract (provisional)

 

Introduction

Fibromyalgia is a chronic pain syndrome with unknown etiology. Recent studies have shown some evidence demonstrating that oxidative stress may have a role in the pathophysiology of fibromyalgia. However, it is still not clear whether oxidative stress is the cause or the effect of the abnormalities documented in fibromyalgia. Furthermore, the role of mitochondria in the redox imbalance reported in fibromyalgia is also controversial. We undertook this study to investigate the role of mitochondrial dysfunction, oxidative stress and mitophagy in fibromyalgia.

Methods

We studied 20 patients (2 males and 18 females) recruited from the database of the Sevillian Fibromyalgia Association and 10 healthy controls. We evaluated mitochondrial function in blood mononuclear cells from fibromyalgia patients measuring coenzyme Q10 levels by high performance liquid chromatography (HPLC), and mitochondrial membrane potential by flow cytometry. Oxidative stress was determined by measuring mitochondrial superoxide production by MitoSOXTM, and lipid peroxidation in blood mononuclear cells and plasma from fibromyalgia patients. Autophagy activation was evaluated by quantifying the fluorescence intensity of LysoTrackerTM Red staining of blood mononuclear cells. Mitophagy was confirmed by measuring citrate synthase activity and electron microscopy examination of blood mononuclear cells.

Results

We found reduced levels of coenzyme Q10, decreased mitochondrial membrane potential, increased level of mitochondrial superoxide in blood mononuclear cells, and increased levels of lipid peroxidation in both blood mononuclear cells and plasma from fibromyalgia patients. Mitochondrial dysfunction was also associated with increased expression of autophagic genes and the elimination of dysfunctional mitochondria by mitophagy.

Conclusions

These findings may support the role of oxidative stress and mitophagy in the pathophysiology of fibromyalgia.

 

Full Text article at : http://arthritis-research.com/content/12/1/R17

 

Also available as PDF document

 

Disclaimer:

Information on this site is provided for educational purposes only and is not meant to substitute the advice provided by your own physician or other medical professionals.
Consult your own physician regarding the applicability of any information listed on this website with respect to your symptoms or medical condition

 

Disclaimer